Composite

Part:BBa_K3002224:Design

Designed by: Marlene Schlosser   Group: iGEM19_TU_Kaiserslautern   (2019-10-21)


L2 spectinomycin resistance + cCA_Mut-PETase_SP20-His + ccA_MHETase_HA-SP20HA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5348
    Illegal PstI site found at 3485
    Illegal PstI site found at 4458
    Illegal PstI site found at 6690
    Illegal PstI site found at 7014
    Illegal PstI site found at 7357
    Illegal PstI site found at 8167
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5348
    Illegal NheI site found at 2665
    Illegal NheI site found at 5612
    Illegal PstI site found at 3485
    Illegal PstI site found at 4458
    Illegal PstI site found at 6690
    Illegal PstI site found at 7014
    Illegal PstI site found at 7357
    Illegal PstI site found at 8167
    Illegal NotI site found at 7025
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5348
    Illegal BglII site found at 7935
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5348
    Illegal PstI site found at 3485
    Illegal PstI site found at 4458
    Illegal PstI site found at 6690
    Illegal PstI site found at 7014
    Illegal PstI site found at 7357
    Illegal PstI site found at 8167
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5348
    Illegal PstI site found at 3485
    Illegal PstI site found at 4458
    Illegal PstI site found at 6690
    Illegal PstI site found at 7014
    Illegal PstI site found at 7357
    Illegal PstI site found at 8167
    Illegal NgoMIV site found at 1401
    Illegal NgoMIV site found at 1584
    Illegal NgoMIV site found at 1694
    Illegal NgoMIV site found at 3220
    Illegal NgoMIV site found at 3247
    Illegal NgoMIV site found at 4949
    Illegal NgoMIV site found at 6623
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is codon-optimized for Chlamydomonas reinhardtii. Additionally, it is compatible with Phytobricks /MoClo parts.


Source

This part is a combination of the parts BBa_K3002102 (spectinomycin resistance), BBa_K3002120 (cCA_Mutant PETase_SP20HA) and BBa_K3002114 (cCA_wildtype MHETase_SP20HA).

References